Journal: Mediators of Inflammation

Article Title: Analysis of Th17 and Tc17 Frequencies and Antiviral Defenses in Gut-Associated Lymphoid Tissue of Chronic HIV-1 Positive Patients

doi: 10.1155/2015/395484

Figure Lengend Snippet: Activation markers, IFN- γ and IL-17A expression in CD4 + and CD8 + T cells subsets in blood and in gut. Panel (a): representative flow cytometry plots outlining CD38 and HLA-DR expression on naïve, effector, and central memory CD4 + and CD8 + lymphocytes derived from peripheral blood mononuclear cells (PBMCs) and gut-associated lymphoid tissue (GALT). Panel (b): representative flow cytometry plots outlining IFN- γ and IL-17A intracitoplasmatic expression on naïve, effector, and central memory CD4 + (Th1 and Th17) and CD8 + (Tc1 and Tc17) lymphocytes derived from PBMCs and GALT.

Article Snippet: Cultured cells were fixed, permeabilized (BD Cytofix/Cytoperm, Becton Dickinson, San Jose, CA, USA), and stained with combinations of fluorochrome-labeled monoclonal antibodies: CD3-PerCP, CD4-APC-Vio770, CD8-FITC, CD45RO-PE-Vio770, CD27-VioBlue, IFN- γ -APC, and IL-17A-PE (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Activation Assay, Expressing, Flow Cytometry, Derivative Assay

Journal: Nature Communications

Article Title: Fasting mimicking diet in mice delays cancer growth and reduces immunotherapy-associated cardiovascular and systemic side effects

doi: 10.1038/s41467-023-41066-3

Figure Lengend Snippet:

Article Snippet: Anti-mouse Granzyme B, FITC (REA226) , Miltenyi Biotec , 130-118-341.

Techniques: In Vivo

Journal: Biomolecules

Article Title: Palmitic Acid Esterification Boosts Epigallocatechin Gallate’s Immunomodulatory Effects in Intestinal Inflammation

doi: 10.3390/biom15081208

Figure Lengend Snippet: Dot plots of representative pro-inflammatory (CD86-Coralite ® Plus 647, M1) and propidium iodide double stains of M0 macrophages (differentiated from THP-1 monocytic cells treated with Phorbol-Myristate Acetate) exposed to pro-inflammatory growth media of Caco-2 cells in the presence/absence of epigallocatechin gallate and palmitoyl epigallocatechin gallate. Distinct lowercase letters indicate significantly different values at p < 0.05 according to one-way analyses of variance (ANOVA) and Duncan’s multiple range test ( n = 3).

Article Snippet: CoraLite ® Plus 647 Anti-Human CD86 and CD206 antibodies were obtained from Proteintech ® (ThermoFisher Scientific, Madrid, Spain; Catalog #CL647-65165 and #CL647-65155, respectively).

Techniques:

Journal: Life sciences

Article Title: TPX2 influences the regulation of macrophage polarization via the NF-κB pathway in lung adenocarcinoma.

doi: 10.1016/j.lfs.2024.122437

Figure Lengend Snippet: Fig.2 Expression of TPX2 in A549 cells affects macrophage polarization. (A) Detection of macrophage surface markers CD80 and CD163 after cell supernatant induction in A549 group, Vehicle group and TPX2-shRNA group. (B) The analysis percentage of macrophage surface marker CD80, CD163; CD80/CD163 after induction by cell supernatant of the three groups. (C) CD80 and CD163 protein expression in macrophages after induction in the supernatant of the three groups cells. (D) The

Article Snippet: THP-1 cell differentiation into macrophages was induced by culturing for 24 h. The supernatant of each group was then applied for 48 h. The induced cells were digested and washed twice, followed by the addition of PE Anti-Human CD80 (B7-1) (PE-65083, Proteintech, China) and APC Anti-Human CD163 (GHI/61) (APC-65169, Proteintech, China) antibodies and resuspended to flow cytometry (BD Biosciences, USA).

Techniques: Expressing, shRNA, Marker